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                天然羥基磷灰石對間充質干細胞作用的分子機理初步研究
                來源: | 作者: | 發布時間:2014-4-15 16:01:48

                天然羥基磷灰石對間充質干細胞作用的分子機理初步研究
                張志偉,王健丹,呂曉迎
                基金項目:中國教育部博士點基金 (No.20100092110027);973計劃項目(No. 2009CB930000);國家自然科學基金 (No. 31170910, 31271012, 30770583);江蘇省研究生培養創新工程基金(CX10B_082Z) 作者簡介:張志偉(1981-),男,博士研究生,主要研究方向:生物材料,生物相容性 通信聯系人:呂曉迎(1956-),女,教授,主要研究方向:生物材料,生物相容性,生物電子學.

                (東南大學生物電子學國家重點實驗室,南京,210096) 5
                摘要:本論文的目的是通過對細胞生物學和生物組學的綜合研究,探討天然羥基磷灰石(NHA)對骨髓間充質干細胞(MSCs)的作用機理;首先從豬骨中提取NHA,并制備成圓盤狀試樣,然后采用細胞生物學方法研究MSCs在NHA圓盤狀試樣表面的增殖情況;接著采用蛋白質組學技術研究培養于NHA表面的MSCs細胞的蛋白表達譜情況,接著進行GO分類分析并與前期10 基因芯片數據的GO分類分析進行比較,最后對蛋白質組學和基因芯片數據進行通路的聯合分析。結果顯示NHA表面培養的MSCs細胞增殖率下降;培養于NHA表面的MSCs細胞的蛋白表達譜所涉及GO功能廣泛,且多數GO功能與基因芯片的GO功能相一致;差異表達的基因/蛋白參與了89條通路,其中MAPK通路可能在NHA誘導MSCs成骨分化中發揮重要作用。總之,NHA對MSCs的作用涉及基因轉錄和蛋白質表達各層次,NHA可能通過對MAPK通路的影15 響,最終誘導了MSCs細胞的成骨分化。
                關鍵詞:生物材料;天然羥基磷灰石;轉錄組學;蛋白質組學;生物學通路
                中圖分類號:R318.08
                Pilot Study of molecular mechanism underlying 20 MSCs–natural hydroxyapatite interaction
                ZHANG Zhiwei, WANG Jiandan, Lü Xiaoying
                (State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096)
                Abstract: This work combined cytobiology and biomics analysis to elucidate the mechanism of the interaction between natural hydroxyapatite (NHA) and mesenchymal stem cells (MSCs). 25 Firstly, NHA power obtained from pig bones was fabricated into disc-shaped sample. Subsequently, the proliferation of MSCs cultured on the disc-shaped NHA was investigated. Then, The proteomics approach was employed to detect protein expression profiling of MSCs cultured on NHA. The biologically relevant gene ontology (GO) terms of differentially expressed proteins was analyzed and compared with GO result of previous transcriptomics data. Finally, an 30 integrating pathway study of proteomics and transcriptomics data was analyzed. The results showed that the cell proliferation rate was decreased on NHA. Protein expression profiling of MSCs cultured on NHA were involved in several GO functional classification. Comparing the GO analysis results from proteomics and gene expression peofile, we found that the differentially expressed genes and proteins shared many GO terms. The genes and proteins involved in 89 35 pathways were shown to be differentially expressed. Among them, MAPK pathways might played an important role in NHA-induced osteogenic differentiation. In conclusion, NHA affected MSCs via both transcription and translation level. NHA-induced osteogenic differentiation of MSCs eventually occurred through the MAPK pathway.
                Key words: Biomaterial, Natural hydroxyapatite, Transcriptomics, Proteomics, Biological 40 pathways

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