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                糖皮質激素誘導大鼠胚胎垂體生長激素細胞分化過程中Ras/ERK 和Ras/PI3K/Akt 的作用
                來源: | 作者: | 發布時間:2013-11-12 15:18:57

                糖皮質激素誘導大鼠胚胎垂體生長激素細胞分化過程中Ras/ERK 和Ras/PI3K/Akt 的作用
                5 程玉1,呂文明1,項瑩2,胡恩喜1,張廣會1,王偉軍1
                基金項目:教育部高等學校博士學科點專項科研基金(編號:20112307120031)
                作者簡介:程玉(1971-),男,副教授,主要研究方向:神經外科學.

                (1. 哈爾濱醫科大學附屬第一醫院神經外科,哈爾濱,150001;2. 哈爾濱醫科大學附屬第二醫院內分泌與代謝科,哈爾濱,150086)
                摘要:目的: 探究糖皮質激素誘導大鼠胚胎垂體生長激素細胞分化過程中Ras/ERK 和Ras/PI3K/Akt 的作用。方法:本課題利用大鼠胚胎垂體細胞的無血清原代細胞培養技術,在糖皮質激素誘導生長激素細胞分化的過程中,加入Ras/ERK 和Ras/PI3K/Akt 信號轉導通路關鍵蛋白Ras、ERK 和Akt 的抑制劑Manumycin、U0126、LY294002,利用免疫熒光、western-blot、放射免疫分析和MTT 等技術對Ras/ERK 和Ras/PI3K/Akt 在糖皮質激素體外誘導生長激素細胞分化中的作用進行研究。結果:糖皮質激素能夠顯著提高生長激素陽性細胞百分比和生長激素的含量(P<0.01); Manumycin、U0126、LY294002 抑制劑處理后,糖皮質激素誘導的生長激素陽性細胞百分比顯著降低(P<0.01),生長激素的含量亦出現降低(P<0.05)。結論:Ras/ERK 和Ras/PI3K/Akt 在糖皮質激素誘導生長激素細胞分化過程中發揮重要作用。
                關鍵詞:基礎醫學;糖皮質激素;生長激素細胞;分化;Ras/ERK;Ras/PI3K/Akt
                中圖分類號:R329.2+1

                Ras/ERK and Ras/PI3K/Akt on glucocorticoids inducing Growth Hormone Cells Differentiation of Fetal Rat in vitro
                Cheng Yu1, Lv Wenming1, Xiang Ying2, Hu Enxi1, Zhang Guanghui1, Wang Weijun1
                (1. Department of Neurosurgery of First Affiliated Hospital,Harbin Medical University,150001;2. Department of Endocrinology of Second Affiliated Hospital,Harbin Medical University,150086)
                Abstract: To explore the function of Ras/ERK and Ras/PI3K/Akt in glucocorticoids induces the differentiation of fetal rat pituitary somatotroph cells in vitro.Methods: This topic uses embryo pituitary cells in vitro serum-free primary cell culture technique. The pituitary was harvested rendered into a fetal pituitary cell suspension. In the experiment Ras ERK and the Akt inhibitors,Manumycin U0126 and LY294002,were added to the suspension for pretreatment and cell culturewas carried out using a medium containing glucocorticoids. After those, immunofluorescence,Western-blot, radioimmunoassay and MTT Technology is used in this experiment.Results:glucocorticoids can increase the percentage of GH-positive cells and GH content (P<0.01).After pretreatment with Manumycin U0126 and LY294002, the percentage of glucocorticoids-induced
                GH-positive cells decreased significantly (P<0.01), and the GH content was also reduced (P<0.05).Conclusion: Ras/ERK and Ras/PI3K/Akt play an important role in the glucocorticoids-induced growth hormone cell differentiation.

                Key words: basic medical;glucocorticoids; GH cell; differentiation;Ras/ERK;Ras/PI3K/Akt

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